Description
Thermo Scientific™ Nalgene™ Rapid-Flow™ Sterile 150 mL Vacuum Filter Units, 0.2 µm PES (565-0020) – Case of 12
The Thermo Scientific™ Nalgene™ 565-0020 is a complete, single-use vacuum filtration system engineered for the sterile purification and storage of small-volume cell culture media, biological fluids, and analytical buffers. Unlike standalone bottle-top filters, this full assembly includes both a 150 mL upper filter funnel and a matching, detachable 150 mL receiver storage bottle. Utilizing Nalgene’s proprietary Rapid-Flow™ support plate system paired with a high-purity polyethersulfone (PES) membrane, it delivers high processing speeds and excellent throughput while providing a certified 0.2 micrometer (µm) biological contamination barrier. Supplied as a commercial case of 12 individually wrapped units, this configuration is a core consumable for benchtop research, tissue culture maintenance, and small-batch fluid preparation.
Technical Performance & Architecture
- Complete Closed-System Assembly: Arrives ready-to-use with the upper funnel and receiver bottle pre-assembled. This eliminates the aseptic docking step required by standard bottle-top components, lowering the risk of touch contamination under the biosafety hood.
- Asymmetric Polyethersulfone (PES) Membrane: Features a 50 mm hydrophilic PES membrane that offers ultra-low protein binding and a low chemical extractable profile. This ensures that essential nutrients, proteins, and growth factors are not pulled out of premium serum or media formulations during filtration.
- Proprietary Rapid-Flow™ Geometry: The internal support infrastructure underneath the membrane optimizes vacuum pressure distribution. This minimizes structural stress on the PES matrix, prevents premature clogging, and reduces overall filtration time.
- Leak-Proof Receiver Storage: The lower 150 mL receiver bottle features deep, high-precision threads and is supplied with a separate, sterile screw cap. Once filtration is complete, the receiver can be safely uncoupled and sealed airtight for secure, long-term cold-room storage.
Product Specifications
- Catalog Number / MPN: 565-0020 (Alternative Ref: 5650020)
- Primary Manufacturer: Thermo Scientific™ (Nalgene™ Filtration Portfolio)
- Volumetric Capacity: 150 mL Upper Funnel / 150 mL Lower Receiver Bottle
- Membrane Pore Size: 0.2 µm sterilizing grade
- Membrane Diameter Profile: 50 mm nominal diameter
- Membrane Material Chemistry: Hydrophilic Polyethersulfone (PES)
- Housing Material Composition: Crystal-clear, impact-resistant polystyrene (PS)
- Sterility & Purity Status: Gamma irradiated (Sterile baseline); Certified Non-Pyrogenic and Non-Cytotoxic
- Packaging Configuration: Case of 12 Complete Units (Each unit individually bagged with a standalone sterile cap, organized within a track-coded master carton)
Key Benefits & Applications
- Small-Batch Media & Serum Prep: The premier choice for sterilizing small aliquots of specialized base media, growth supplements, antibiotics, or fetal bovine serum (FBS) without wasting hardware capacity.
- Downstream Protein and Enzyme Purification: Provides an inert, low-binding flow path for clarifying protein extracts, chromatography buffers, and enzyme solutions without losing precious product yield to membrane retention.
- Ergonomic Quick-Connect Port: Features an integrated vacuum port with a universal quick-disconnect arm. This setup keeps the vacuum line secure and stable, while the broad base profile prevents accidental tipping on the benchtop.
- **cGMP Inventory Compliance:** The plastic housing is directly printed with clear volumetric graduations, the catalog number, manufacturing lot code, and membrane details to allow simple, audit-ready batch traceability.
Thermo Scientific™, Nalgene™, and Rapid-Flow scraps are registered commercial trademarks of Thermo Fisher Scientific Inc. Catalog number 565-0020 specifies the 150 mL complete filter unit assembly with a 0.2 µm PES membrane. For filtering larger volumes or using aggressive organic solvents that require alternative membranes like Nylon or Cellulose Acetate, larger 500 mL/1000 mL profiles or alternative polymer configurations should be selected.
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