Description
Description
Rpmi-1640 Mediumdutch Modification With Sodium Bicarbonate And 20Mm Hepes 500mL Case/6
- MDL number MFCD00217820
- NACRES NA.75
| Quality Level | 300 |
| sterility | sterile-filtered |
| form | liquid |
| application(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| pH range | >7.2 |
| components | sodium pyruvate: noL-glutamine: noNaHCO3: yesphenol red: yesHEPES: 20 mM |
| shipped in | ambient |
| storage temp. | 2-8°C |
Application
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
Reconstitution
Supplement with 0.3 g/L L-glutamine.
Documents
Safety Data Sheet
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