Description
Description
HEPES Sodium Salt Solution Bioreagent 1M 500mL
- CAS Number 75277-39-3
- Empirical Formula (Hill Notation) C8H17N2NaO4S
- Molecular Weight 260.29
- MDL number MFCD00036463
- PubChem Substance ID 24895590
- NACRES NA.25
| Quality Level | 200 |
| sterility | sterile-filtered (0.2 μm filtered) |
| product line | BioReagent |
| application(s) | cell culture | mammalian: suitable |
| impurities | Bioburden, tested |
| DNase, RNase. Protease, Nickase, free | |
| endotoxin, tested | |
| cation traces | Fe: |
| SMILES string | [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O |
| InChI | 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1 |
| InChI key | RDZTWEVXRGYCFV-UHFFFAOYSA-M |
General description
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Application
HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.
Documents
Safety Data Sheet
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