Description
Description
Hepesbioxtra Ph 5.0-6.5 1 M In H2O ≥99.5% Titration 50 Gram
Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
- CAS Number 7365-45-9
- Empirical Formula (Hill Notation) C8H18N2O4S
- Molecular Weight 238.30
- Beilstein/REAXYS Number 883043
- EC Number 230-907-9
- MDL number MFCD00006158
- PubChem Substance ID 24895796
| Quality Level | 400 |
| product line | BioXtra |
| assay | ≥99.5% (titration) |
| impurities | Insoluble matter, passes filter test |
| ign. residue (900°C) | ≤0.1% (as SO4) |
| loss | ≤1.0% loss on drying, 110°C |
| pH | 5.0-6.5 (1 M in H2O) |
| useful pH range | 6.8 – 8.2 |
| pKa (25 °C) | 7.5 |
| solubility | H2O: 1 M, clear, colorless |
| anion traces | chloride (Cl-): ≤0.05% |
| cation traces | Al: ≤0.0005% |
| As: ≤0.0001% | |
| Ba: ≤0.0005% | |
| Bi: ≤0.0005% | |
| Ca: ≤0.001% | |
| Cd: ≤0.0005% | |
| Co: ≤0.0005% | |
| Cr: ≤0.0005% | |
| Cu: ≤0.0005% | |
| Fe: ≤0.0005% | |
| K: ≤0.005% | |
| Li: ≤0.0005% | |
| Mg: ≤0.0005% | |
| Mn: ≤0.0005% | |
| Mo: ≤0.0005% | |
| Na: ≤0.005% | |
| Ni: ≤0.0005% | |
| Pb: ≤0.0005% | |
| Sr: ≤0.0005% | |
| Zn: ≤0.0005% | |
| absorption | ≤0.035 at 280 in H2O at 1 M |
| ≤0.045 at 260 in H2O at 1 M | |
| Featured Industry | Diagnostic Assay Manufacturing |
| SMILES string | OCCN1CCN(CC1)CCS(O)(=O)=O |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used:
• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.
Documents
Safety Data Sheet
Reviews
There are no reviews yet.