Description
Description
Bovine Serum Albumin Heat Shock Fraction Essentially Globulin Free pH 7 ≥98%, 50 Gram
Synonym: Albumin bovine serum, BSA, Bovine albumin
- CAS Number 9048-46-8
- EC Number 232-936-2
- MDL number MFCD00130384
- NACRES NA.27
| Quality Level | 200 |
| biological source | bovine |
| assay | ≥98% |
| form | lyophilized powder |
| mol wt | ~66 kDa |
| purified by | heat shock fractionation |
| origin | USA origin |
| free fatty acid content | ≤0.02% |
| application(s) | ELISA: suitable |
| blood typing: suitable | |
| ligand binding assay: suitable | |
| impurities | essentially globulin free |
| loss | ≤5% |
| pH | 7 |
| solubility | water: soluble (40 mg/ml) |
| foreign activity | protease ≤0.005 U/mg |
| storage temp. | 2-8°C |
General description
Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
This BSA product is specifically evaluated and tested for the following properties:
• Very low protease content / protease-free
• Very low fatty acid content / fatty acid-free
Protease-free BSA is especially useful for applications like:
• Enzyme assays
• Protein-based assays
Protease-sensitive techniques that include:
a. Enzyme immunoassay (EIA)
b. Nucleic acid hybridization
c. adioimmunoassay (RIA)
Application
Bovine Serum Albumin has been used:
• as a constituent of medium used for the differentiation of human pluripotent stem cells
• in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA
• to permeabilize fetal cortical cells
Safety Information
Personal Protective Equipment Eyeshields, Gloves
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS MT6446000
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