BIA Separations CIMac Analytical Column 0.1 ml 6 um

Description

Sartorius BIA Separations CIMac QA Strong Anion-Exchange Monolithic Analytical Column – 0.1 mL Bed Volume, 6 µm Channel Size, Quaternary Amine Ligand, Process Analytical Technology (PAT) Tool (MPN: 110.5113-6)

The Sartorius BIA Separations 110.5113-6 is an instrument-grade, high-performance Convective Interaction Media (CIM®) monolithic analytical column engineered for rapid, high-resolution HPLC monitoring, fingerprinting, and quantitative in-process control of large biomolecules. Featuring a compact 0.1 mL bed volume with optimized 6-micrometer (µm) flow-through channels, this column utilizes a covalently bound quaternary amine (QA) functional chemistry to act as a highly efficient strong anion exchanger. Because its convective mass transport mechanism makes its binding capacity completely independent of flow rate, the column allows bioprocess engineers to run fast analysis cycles at high volumetric flow velocities (up to 30 CV/min). This ensures quick quantity and purity feedback on target analytes like viral vectors, pDNA, exosomes, and large proteins in just minutes, making it an essential tool for Process Analytical Technology (PAT) workflows.

Technical Performance and Design

• Convective Mass Transport Monolith: Built with a single-block polymethacrylate matrix featuring interconnected 6 µm channels. Fluid flows through the channels via convective transport rather than slow diffusion, allowing high-velocity runs without loss of resolution or backpressure spikes.

• Quaternary Amine Strong Anion Chemistry: Outfitted with permanently charged QA ligands that maintain a strong positive charge across a broad working pH range of 2 to 13. This chemistry ensures reliable binding and high-capacity retention of negatively charged biomolecules.

• Flow-Independent Analytical Resolution: Enables ultra-fast analytical runs spanning flow rates from 0.2 to 3 mL/min (2 to 30 Column Volumes per minute). This design allows technicians to complete high-accuracy separation gradients in under 5 minutes.

• Aggressive 1 M NaOH Sanitization: Highly stable polymer frame allows for complete, aggressive Clean-In-Place (CIP) flushes using 1 M Sodium Hydroxide. This capability ensures thorough endotoxin removal and eliminates sample carryover between different processing lots.

Product Specifications

• Catalog Number / MPN: 110.5113-6

• Manufacturer / Brand: Sartorius BIA Separations

• Column Series: CIMac™ QA Analytical Series

• Total Bed Volume: 0.1 mL (Micro-Analytical Scale Dimensions)

• Monolith Channel Size: 6 µm (6 micrometers)

• Chromatography Mode: Strong Anion-Exchange (AEX)

• Functional Group Ligand: QA (Quaternary Amine)

• Operational Flow Rate Range: 0.2 to 3.0 mL/min (1 to 15 cm/min linear velocity)

• Maximum Safe Pressure Threshold: 16 MPa (150 bar / 2,175 psi)

• Fluidic Connector Interfaces: Standard 10-32 UNF coned ports matching 1/16-inch OD capillary tubing lines

• Housing Assembly Material: Precision-engineered, passivated Stainless Steel body casing

Key Benefits and Applications

• In-Process Bioprocess Monitoring (PAT): The industry-preferred at-line testing standard for tracking mass balances, downstream fraction purities, and recovery yields directly from active cell culture bioreactors.

• Viral Vector and Vaccine Analytics: Extensively utilized across gene therapy networks to cleanly isolate and quantify complex targets, including Adenoviruses, Lentiviruses, orf viruses, and larger virus-like particles (VLPs).

• Endotoxin and Host DNA Screening: Serves as a highly reliable diagnostic filter to monitor trace endotoxin clearances and verify low genomic host DNA carryover profiles across purification steps.

• Low-Shear Exosome Protection: The gentle, laminar flow path within the 6 µm channels prevents structural shearing of fragile extracellular vesicles, target IgM antibodies, and large RNA payloads, ensuring high biological recovery rates.

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